BBa_K1321334

BBa_K1321334 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1321334
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Laura de Arroyo Garcia
Date created: 2014-10-07 11:00:00
Date modified: 2015-05-08 01:09:53

pSB-AcsAB



Types
DnaRegion

Roles
Translational_Unit

engineered_region

Sequences BBa_K1321334_sequence (Version 1)

Description

This part is made up of the coding sequence of the A and B elements of the acs, cellulose-producing operon. AcsA and acsB are transcribed and translated in the form of a single polypeptide, and form the main two domains (regulatory and catalytic) of the Cellulose Synthase enzyme in Gluconacetobacter Xylinus.

Notes

The Ribosomal binding site of AcsAB is already included within the sequence. Because we are transferring this element from Gluconacetobacter into simpler organisms, such as E.coli, the native RBS was replaced with a new RBS, that would guarantee strong translation whilst maintaining the native stoichiometry for the final operon system to operate successfully.

Source

The AcsAB coding sequence was extracted from NCBI, codon optimised and edited accordingly for further gene synthesis and cloning into pSB1C3.

Sequence Annotation Location Component / Role(s)
RBS - AcsAB
AcsAB Coding Sequence
Double Stop Codon
Start Codon
28,51
68,4678
4679,4684
68,71
ribosome_entry_site feature/rbs
CDS feature/cds
feature/stop stop_codon
start_codon feature/start
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1321334/1