Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ophir Ospovat
Date created: 2014-10-14 11:00:00
Date modified: 2015-05-08 01:09:53
Self-Regulating Oscillating Fluorescent System
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1322001_sequence (Version 1) |
Description
See BBa_K546546 for function. This part was optimized through site-directed mutagenesis of specific restriction sites in order to make the original part RFC[21] and RFC[25] compatible.Notes
N/ASource
N/A| Sequence Annotation | Location | Component / Role(s) |
| RBS RBS RBS RBS LVA LVA LVA AHL Autoinducer Synthetase Autoinducer Inactivation Enzyme A GFP Constitutive Promoter LuxR Repressor/Activator LuxR/HSL Regulated Promoter pLux/Lac Hybrid Promoter pLux/Tet Hybrid Promoter T1 Terminator T1 Terminator T1 Terminator T1 Terminator TE Terminator TE Terminator TE Terminator TE Terminator | 44,55 1068,1079 2127,2138 2996,3007 1836,1868 2724,2756 3734,3766 2145,2787 1086,1899 3014,3772 1,35 62,842 2933,2987 2045,2118 988,1059 851,930 1908,1987 2796,2875 3781,3860 939,979 1996,2036 2884,2924 3869,3909 | feature/rbs ribosome_entry_site feature/rbs ribosome_entry_site ribosome_entry_site feature/rbs ribosome_entry_site feature/rbs feature/tag tag tag feature/tag tag feature/tag feature/protein CDS CDS feature/protein CDS feature/protein promoter feature/promoter CDS feature/protein feature/promoter promoter feature/promoter promoter promoter feature/promoter stop_codon feature/stop stop_codon feature/stop stop_codon feature/stop stop_codon feature/stop stop_codon feature/stop feature/stop stop_codon feature/stop stop_codon feature/stop stop_codon |