BBa_K1323017

BBa_K1323017 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1323017

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Julia Manalil

Date created: 2014-10-03 11:00:00

Date modified: 2015-05-08 01:09:54

E. coli - Staphylococcus Shuttle Vector

Details

• Types: DnaRegion
• Roles: Plasmid_Backbone; plasmid_vector
• Sequences: BBa_K1323017_sequence (Version 1)

Description

TBA

Notes

TBA

Source

TBA

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Biobrick suffix	1,21	feature/dna, sequence_feature
E. coli his operon terminator	22,93	stem_loop, feature/stem_loop
Stem loop	30,64	stem_loop, feature/stem_loop
Verification reverse (VR) primer binding site	157,176	primer_binding_site, feature/primer_binding
Scar: BamHI sites used to insert parts	178,191	feature/scar
oriV (Staphylococcal ORI from pSK41)	192,2124	operator, feature/operator
Erythromycin resistance	2172,2903	feature/cds, CDS
Scar: BglII site used to insert parts	2978,2983	feature/scar
rep (pMB1)	3066,3680	feature/misc, sequence_feature
ORI	3081,3081	sequence_feature, feature/misc
T0 Terminator	3847,3952	stem_loop, feature/stem_loop
Chloramphenicol resistance	3966,4625	feature/cds, CDS
Verification forward (VF2) primer binding site	4738,4757	primer_binding_site, feature/primer_binding
end of terminator	4814,4817	feature/stem_loop, stem_loop
Stem loop	4827,4846	feature/stem_loop, stem_loop
Biobrick prefix	4855,4876	sequence_feature, feature/dna
BBa_J04450	4877,5945	engineered_region, feature/BioBrick

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1323017/1

Member of these Collections

• iGEM Parts Registry
• igem_plasmid_vectors