Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tianyi Li
Date created: 2014-10-08 11:00:00
Date modified: 2015-05-08 01:09:54
HXT1-CMVmini
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K1328000_sequence (Version 1) |
Description
Glucose-regulated eukaryotic promoter sequence, activated under high glucose concentrations. Works in yeast as well as human fibroblast cells. HXT1 encodes a yeast hexose transporter, we fused its promoter sequence (-750 ~ -161 bp) to a CMV-mini promoter sequence (204 bp) to ensure glucose-sensitivity as well as high expression rates.Ferrer-Mart??nez A, Riera A, Jim??nez-Chillar??n JC, Herrero P, Moreno F, G??mez-Foix AM. A glucose response element from the S. cerevisiae hexose transporter HXT1 gene is sensitive to glucose in human fibroblasts. J Mol Biol. 2004 May 7;338(4):657-67. PubMed PMID: 15099735.
Notes
Region -750~-161 bp was selected from the HXT1 promoter for maximal glucose sensitivity. A CMV-mini promoter sequence was added to increase expression rate.Source
HXT1 promoter sequence is cloned from S. cerevisiae genome via PCR. The CMV-mini promoter is cloned from a commercial plasmid.| Sequence Annotation | Location | Component / Role(s) |
| HXT-1 Promoter CMV-mini Promoter | 1,586 587,790 | promoter feature/promoter promoter feature/promoter |