Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lingfeng Hou
Date created: 2014-10-08 11:00:00
Date modified: 2015-05-08 01:09:54
HXT1-CMVmini-EGFP-tADH1
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1328002_sequence (Version 1) |
Description
This is a composite part containing a glucose-sensitive promoter (BBa_K1328000) and a EGFP reporter with a tADH1 transcription terminator.S. cerevisiae cells transformed with this part was cultured in glucose-free medium with glycerol as carbon-source for 8 hrs. They were then cultured for an additional hour with varying glucose levels (0, 5, 15, 25 mmol/L). A increasing level of GFP expression was observed with increasing levels of glucose concentration, in both microscopy and flow cytometry observations.
Notes
Part sequence contains EcoRI and PstI restriction sites, consider using SpeI and XbaI for cloning.Source
Promoter region is assembled from parts: HXT1-CMVmini promoter (BBa_K1328001), EGFP (BBa_K1123017) and tADH1 transcription terminator.| Sequence Annotation | Location | Component / Role(s) |
| HXT1 Promoter CMV-mini Promoter EGFP tADH1 terminator | 1,586 587,790 840,1556 1561,1748 | promoter feature/promoter promoter feature/promoter CDS feature/protein feature/stop stop_codon |