Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lingfeng Hou
Date created: 2014-10-08 11:00:00
Date modified: 2015-05-08 01:09:54
Insulin (modified protease recognition sites)
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1328003_sequence (Version 1) |
Description
Encodes the human insulin gene (full length). Two 6 bp sequences were inserted into two separate sites within the insulin gene to allow enzymatic digestion by the protease furin. Originally, proinsulin is cleaved three times with three different enzymes, prohormone convertases (PC1 and PC2), and carboxypeptidase E before being secreted as mature insulin. Since PC1 and PC2 expression is tissue specific, we replaced their recognition sites with that of furin, which is universally expressed, thus allowing this insulin gene to be expressed and processed in any somatic cell type.Notes
PC1 and PC2 protease recognition sites were replaced with that of furin, to allow insulin processing in somatic cells other than natural insulin-secreting cells.Source
Assembled from 12 short synthesized DNA sequences.| Sequence Annotation | Location | Component / Role(s) |
| Insulin CDS Furin site Furin site Regulator | 43,384 205,216 310,321 1,42 | feature/cds CDS feature/mutation sequence_alteration sequence_alteration feature/mutation sequence_feature feature/misc |