BBa_K1329001

BBa_K1329001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1329001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Daniela Geist

Date created: 2014-09-30 11:00:00

Date modified: 2015-05-08 01:09:54

Hag-KpnI

Details

• Types: DnaRegion
• Roles: engineered_region; Device
• Sequences: BBa_K1329001_sequence (Version 1)

Description

Hag-KpnI consists of the Bacillus subtilis flagellin gene which as designed with a KpnI site to integrate different domains, tags or modules with a Gibson assembly after linearizing the vector with KpnI. Using a shuttle vector for gam-positive bacteria it is possible to integrate the modified flagellin construct into the Bacillus genome considering a special heat shock protocoland specific flanks besides the used gene locus.
By engineering new modules into the conserved structure of flagellin Bacillus is able to form flagella build up from those modified flagellins.

Notes

The position of the KpnI site had to be well placed in order to provide misfolding and structural problems of the flagellin which could influence the assembly of the filament.

Source

The sequence for this part comes from the Bacillus subtilis genome.

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1329001/1

Member of these Collections

• iGEM Parts Registry