BBa_K1329002

BBa_K1329002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1329002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Daniela Geist

Date created: 2014-10-06 11:00:00

Date modified: 2015-05-08 01:09:54

Insertion of the Metallothionein Cup-1-1 domain into Bacillus subtilis flagellin

Details

• Types: DnaRegion
• Roles: engineered_region; Device
• Sequences: BBa_K1329002_sequence (Version 1)

Description

The bacterial flagellum is built up of multiple copies of a protein called flagellin. The structure of this protein is mainly conserved between different species, but the variation of a loop directed to the outside of the flagellum can be observed. As we aim to fish metal ions from solution the bacterial flagellum was chosen as a protein scaffold system. We inserted the metallothionein Cup1-1 from Saccharomyces cerevisiae into the variable loop of B. subtilis flagellin. Cup1-1 can bind up to 7 ions of either copper or silver.

Notes

GGSS-linkers for better exposition to the outside of the flagellin

Source

Bacillus subtilis
Saccharomyces cerevisiae

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1329002/1

Member of these Collections

• iGEM Parts Registry