BBa_K133000

BBa_K133000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K133000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Vid Kocar
Date created: 2008-10-29 12:00:00
Date modified: 2015-05-08 01:09:55

CF-mCherry-RGD-Hisstop



Types
DnaRegion

Roles
engineered_region

Intermediate

Sequences BBa_K133000_sequence (Version 1)

Description

Part consists of eingeneered chimeric flagellin with Red Flourescent Protein - Cherry, that serves for detecting chimeric protein localization in cells. RGD peptide is added for better internalization.

Notes

Our team had to select the most appropriate region of the conserved region in E. coli flagellin and hyper variable region of H. pylori.

Source

Chimeric protein is a fusion protein of H. pylori and E. coli flagellin. DNA was obtained from genomic DNA with polimerase chain reaction.

igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K133000/1