RGD-His-St

BBa_K133036 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K133036

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jerneja Mori

Date created: 2008-10-25 11:00:00

Date modified: 2015-05-08 01:09:55

RGD-His affinity tag-Stop

Details

• Types: DnaRegion
• Roles: tag; Tag
• Sequences: BBa_K133036_sequence (Version 1)

Description

This is His-tag with stop codon and RGD seguence. His-tag is used for detection of His-tagged proteins by anti-His antibodies. RGD is arginine-glycine-aspartic amino acid sequence, present on several extracellular matrix proteins. It is required for binding to integrins.

Notes

Cloned into pSB1AK3 vector.

Source

Part was constructed by primer annealing.

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K133036/1

Member of these Collections

• //proteindomain/affinity
• //proteindomain/tail
• iGEM Parts Registry