Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yanwen Xie
Date created: 2014-09-22 11:00:00
Date modified: 2015-05-08 01:09:57
lambda cI-LGF2(E75A)
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1333200_sequence (Version 1) |
Description
Fuse lambda cI with LGF2 mutated from Glu to Ala at site 75 (the Glu which locates after three continuous Ala behind lambda repressor is at site 68). As one of the components in Bacterial Two-hybrid System, it causes little interaction with GAL11P compared to the normalized WT.Notes
The LGF2 is mutated from GAA to GCA at site 75.Source
It is from Agilent Technologies' BacterioMatch ⅡTwo-Hybrid System Vector Kit and modified by Site-Directed Mutagenesis(SDM).| Sequence Annotation | Location | Component / Role(s) |
| lambda cI ATG start codon LGF2(E75A) TGA stop codon | 1,711 1,3 721,840 841,843 | CDS feature/protein feature/start start_codon CDS feature/protein stop_codon feature/stop |