BBa_K1334017

BBa_K1334017 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1334017
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Qiushuang Wu
Date created: 2014-09-26 11:00:00
Date modified: 2015-05-08 01:09:58

PADH from Pseudomonas putida



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K1334017_sequence (Version 1)

Description

PADH from Pseudomonas putida is a formaldehyde dehydrogenase which can oxidize free formaldehyde to formate independent of GSH.This part is the coding sequence for the enzyme PADH

Notes

The original sequence contains a Not1 site,by point mutation,we removed the site

Source

Using the genomic DNA of P. putida as the template, the coding regions of the PADH gene (padh) was amplified by PCR. The PCR products were then subcloned into PSB1C3

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BBa_K1334017/1