BBa_K1334017

BBa_K1334017 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1334017

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Qiushuang Wu

Date created: 2014-09-26 11:00:00

Date modified: 2015-05-08 01:09:58

PADH from Pseudomonas putida

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K1334017_sequence (Version 1)

Description

PADH from Pseudomonas putida is a formaldehyde dehydrogenase which can oxidize free formaldehyde to formate independent of GSH.This part is the coding sequence for the enzyme PADH

Notes

The original sequence contains a Not1 site,by point mutation,we removed the site

Source

Using the genomic DNA of P. putida as the template, the coding regions of the PADH gene (padh) was amplified by PCR. The PCR products were then subcloned into PSB1C3

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1334017/1

Member of these Collections

• iGEM Parts Registry