Types | DnaRegion
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Roles | Coding
CDS
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Sequences | BBa_K1334017_sequence (Version 1)
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Description
PADH from Pseudomonas putida is a formaldehyde dehydrogenase which can oxidize free formaldehyde to formate independent of GSH.This part is the coding sequence for the enzyme PADH
Notes
The original sequence contains a Not1 site,by point mutation,we removed the site
Source
Using the genomic DNA of P. putida as the template, the coding regions of the PADH gene (padh) was amplified by PCR. The PCR products were then subcloned into PSB1C3