| Types | DnaRegion
|
| Roles | CDS
Coding
|
| Sequences | BBa_K1336001_sequence (Version 1)
|
Description
A gene (612bp) coding for a non-specific enzyme from Pseudomonas putida that is used to degrade a double nitrogen (N=N) azo bond, as found in azo dyes. It is heat-stable AzoR mutant (BBa_K1336000).
Notes
When designing PCR primers to change the AzoR gene into the BioBrick format, we also had to design primers for site-directed mutagenesis, as there are 2 illegal PstI restriction sites within the native gene (at positions 89 and 170).
Source
From genomic sequence of Pseudomonas putida; AzoR gene inserted into a pET-21a (+) expression vector, provided to us by XXX Research Group.
