BBa_K1336004

BBa_K1336004 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1336004
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yan-Kay Ho
Date created: 2014-10-05 11:00:00
Date modified: 2015-05-08 01:09:58

Dye-decolourising peroxidase from Pseudomonas putida



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K1336004_sequence (Version 1)

Description

PpDyP gene (861bp) from Pseudomonas putida is a relatively novel gene, but which appears to be able to oxidise a wide variety of substrates at high efficiency, like that of azo dyes, anthraquinones, phenolic compounds, manganese, or veratryl alcohol.

Notes

When designing PCR primers to change the PpDyP gene into the BioBrick format, we also had to design primers for site-directed mutagenesis, as there is 1 illegal restriction site within the native gene: EcoRI at position 72.

Source

From genomic sequence of Pseudomonas putida; PpDyP gene inserted into a pET-21a (+) expression vector, provided to us by XXX Research Group.

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BBa_K1336004/1