Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yan-Kay Ho
Date created: 2014-10-05 11:00:00
Date modified: 2015-05-08 01:09:58
Antisense for octaprenyl diphosphate synthase (ispB gene)
| Types | DnaRegion |
| Roles | Other sequence_feature |
| Sequences | BBa_K1336005_sequence (Version 1) |
Description
An antisense fragment designed using the sense/coding strand of the ispB gene as a method for gene knockdown (i.e. as RNAi) of a crucial stage in the production of quinones within E. coli metabolic processes.Notes
When designing PCR primers to change the reverse complement sequence of the ispB gene into the BioBrick format, we truncated the fragment to avoid an illegal PstI restriction site (and so no extra primers were required for site-directed mutagenesis to remove illegal sites).Source
From genomic sequence of Escherichia coli.| Sequence Annotation | Location | Component / Role(s) |
| antisense ispB coding region | 1,562 | CDS feature/cds |