Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yan-Kay Ho
Date created: 2014-10-05 11:00:00
Date modified: 2015-05-08 01:09:58
LacI Expression Cassette with ispB RC gene
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1336006_sequence (Version 1) |
Description
An antisense fragment designed using the sense/coding strand of the ispB http://parts.igem.org/Part:BBa_K1336005 gene as a method for gene knockdown (i.e. as RNAi) of a crucial stage in the production of quinones within E. coli metabolic processes. At the 5' end, there is also a LacI expression cassette http://parts.igem.org/Part:BBa_K314103 IPTG-inducible promoter to drive ispB expression.Notes
When designing PCR primers to change the reverse complement sequence of the ispB gene into the BioBrick format, we truncated the fragment to avoid an illegal PstI restriction site (and so no extra primers were required for site-directed mutagenesis to remove illegal sites).Source
From genomic sequence of Escherichia coli.| Access | Instance | Definition |
| public public | BBa_K314103 BBa_K1336005 | BBa_K314103 BBa_K1336005 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K314103 BBa_K1336005 | 1,1638 1647,2208 | BBa_K314103 BBa_K1336005 |