BBa_K1343004

BBa_K1343004 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1343004

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Tal Naor, Ricarina Rabinovitz, Ittai Rubinstein, Yair Maimon

Date created: 2014-09-26 11:00:00

Date modified: 2015-05-08 01:09:59

Plac-CI-T7

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K1343004_sequence (Version 1)

Description

lambda phage repressor (CI) and T7 RNA Polymerase under LacI regulated promoter

Notes

To assamble this part, and others like it, we used a specialized Gibson Barcode System to allow for a modular construction of gates with various components using the same Gibson reaction conditions, allowing for the assembly of a large number of constructs in parallel to each other

Source

Placeholder

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Barcode Label 1	135,156	sequence_feature, feature/misc
Plac	1,112	promoter, feature/promoter
Barcode Label 4	157,178	feature/misc, sequence_feature
Barcode Label 6	113,134	feature/misc, sequence_feature
BBa_B0030: Strong RBS	179,193	feature/rbs, ribosome_entry_site
CI lambda repressor	200,913	feature/protein, CDS
Barcode Label 3	914,935	sequence_feature, feature/misc
BBa_B0030: Strong RBS	936,950	feature/rbs, ribosome_entry_site
T7 terminator	3755,3801	stop_codon, feature/stop
Barcode Label 5	3653,3674	sequence_feature, feature/misc
Barcode Label 2	3631,3652	feature/misc, sequence_feature
BBa_B0010:T1 (terminator) from E. coli	3675,3754	stop_codon, feature/stop
Barcode Label 4	3609,3630	feature/misc, sequence_feature
BBa_K145001:T7 RNA polymerase (BL21DE3)	957,3608	feature/protein, CDS

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1343004/1

Member of these Collections

• iGEM Parts Registry