BBa_K1343007

BBa_K1343007 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1343007
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tal Naor, Ricarina Rabinovitz, Ittai Rubinstein, Yair Maimon
Date created: 2014-09-26 11:00:00
Date modified: 2015-05-08 01:09:59

Ptet-CI



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_K1343007_sequence (Version 1)

Description

lambda phage CI repressor protein under tetR regulated promoter

Notes

To assamble this part, and others like it, we used a specialized Gibson Barcode System to allow for a modular construction of gates with various components using the same Gibson reaction conditions, allowing for the assembly of a large number of constructs in parallel to each other

Source

Placeholder

Sequence Annotation Location Component / Role(s)
BBa_R0040:TetR repressible promoter
barcode 6
barcode 1
barcode 4
BBa_B0030: Strong RBS
CI lambda repressor
barcode 3
barcode 2
barcode 5
BBa_B0010:T1 (terminator) from E. coli
T7 terminator
1,54
55,76
77,98
99,120
121,135
142,855
856,877
878,899
900,921
922,1001
1002,1048
feature/promoter promoter
feature/barcode engineered_tag
engineered_tag feature/barcode
engineered_tag feature/barcode
ribosome_entry_site feature/rbs
CDS feature/protein
feature/barcode engineered_tag
feature/barcode engineered_tag
feature/barcode engineered_tag
feature/stop stop_codon
feature/stop stop_codon
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1343007/1