BBa_K1343008

BBa_K1343008 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1343008

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Tal Naor, Ricarina Rabinovitz, Ittai Rubinstein, Yair Maimon

Date created: 2014-09-26 11:00:00

Date modified: 2015-05-08 01:09:59

Ptet-LacI

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K1343008_sequence (Version 1)

Description

LacI repressor protein under tetR regulated promoter

Notes

To assamble this part, and others like it, we used a specialized Gibson Barcode System to allow for a modular construction of gates with various components using the same Gibson reaction conditions, allowing for the assembly of a large number of constructs in parallel to each other

Source

Placeholder

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0040:TetR repressible promoter	1,54	feature/promoter, promoter
barcode 6	55,76	engineered_tag, feature/barcode
barcode 1	77,98	engineered_tag, feature/barcode
barcode 4	99,120	feature/barcode, engineered_tag
BBa_B0030: Strong RBS	121,135	feature/rbs, ribosome_entry_site
lacI	142,1233	feature/protein, CDS
barcode 3	1234,1255	feature/barcode, engineered_tag
barcode 2	1256,1277	feature/barcode, engineered_tag
barcode 5	1278,1299	engineered_tag, feature/barcode
BBa_B0010:T1 (terminator) from E. coli	1300,1379	stop_codon, feature/stop
T7 terminator	1380,1427	stop_codon, feature/stop

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1343008/1

Member of these Collections

• iGEM Parts Registry