BBa_K1343016

BBa_K1343016 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1343016

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Karen Joseph

Date created: 2014-10-04 11:00:00

Date modified: 2015-05-08 01:09:59

Pcat->RBS->TaZ->dTer

Details

• Types: DnaRegion
• Roles: Generator; engineered_region
• Sequences: BBa_K1343016_sequence (Version 1)

Description

This part generates the TaZ receptor. The TaZ is under a Pcat constitutive promoter.
TaZ is a fused Tar-EnvZ receptor meant for Aspartate dependent response, by activating the EnvZ/OmpR two component signalling system and thus activating the PompC promoter.
We used this part for prove-of-concept for low concentration detection. By using this TaZ Generator we were able to test different concentrations of Aspartate and their effect on the PompC promoter present in another plasmid.

Notes

We had to add a gggggg sequance between RBS and TaZ sequances.

Source

Pcat: BBa_I14033
RBS: BBa_B0034
TaZ: BBa_C0082
dTer: BBa_B0015

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Pcat	1,38	feature/promoter, promoter
RBS	39,50	ribosome_entry_site, feature/rbs
Taz	57,1572	feature/misc, sequence_feature
dTer	1765,1893	sequence_feature, feature/misc

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1343016/1

Member of these Collections

• //classic/signalling/sender
• iGEM Parts Registry