Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Oliver Purcell
Date created: 2008-10-19 11:00:00
Date modified: 2015-05-08 01:10:01
pCpxR (CpxR responsive promoter)
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K135000_sequence (Version 1) |
Description
This is suggested to be an adhesion responsive promoter. It contains the binding site for CpxR-P (phosphorylated CpxR). CpxR is one member of the two-component Cpx response to envelope stress. Briefly, envelope stress causes CpxA, an inner-membrane histidine Kinase to Phosphorylate CpxR, allowing it to bind to CpxR-P responsive regions and regulate downstream target genes. See 'Surface sensing and adhesion of Escherichia coli controlled by the Cpx-signaling pathway', Karen Otto and Thomas J. Silhavy, PNAS, Vol. 99., No, 4., 2287-2292. 2002.Notes
NoneSource
This part comes from the genome of E.coli TR235, as used in the PNAS paper of Otto and Silhavy mentioned previously. TR235 is K-12 MC4100 containing a pCpxR-LacZ fusion inserted at the (Lambda)att site, plus some other modifications (see Otto and Silhavy). The part is a portion of the CpxR responsive region used in these LacZ fusions.| Sequence Annotation | Location | Component / Role(s) |
| CpxR-P Binding site BBa_K135000 | 1,15 1,55 | non_covalent_binding_site feature/binding promoter feature/promoter |