BBa_K1351004

BBa_K1351004 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1351004

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Mona Dotzler

Date created: 2014-10-04 11:00:00

Date modified: 2015-06-01 08:15:05

SigD-dependent promoter of the LytABC locus (PlytA) of Bacillus subtilis

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K1351004_sequence (Version 1)

Description

PlytA promoter of the B. subtilis LytABC operon, under the control of SigD (major), the transcripts being predominants at the exponential growth phase, and SigA (minor).

Notes

to be filled

Source

This part was generated by amplification from B. subtilis W168 gDNA with the primers listed below, followed by digestion with EcoRI and PstI and ligation into pSB1C3.

PlytA_ENX_fwd: TGCAGAATTCGCGGCCGCTTCTAGAG ctcatcctttgcacctcg
PlytA_SNP_rev: AGCCTGCAGCGGCCGCTACTAGTA tcattatattttatcgtcaacctatt

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
-35	143,146	feature/promoter, promoter
-10	161,168	feature/promoter, promoter

Other Properties

• igem#experience: None
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1351004/1

Member of these Collections

• //chassis/prokaryote/bsubtilis
• //regulation
• iGEM 2018 Distribution Plate 5
• iGEM 2018 Distribution Plate 5 Well 2F
• iGEM 2018 Part Library
• iGEM 2018 Plasmid pSB1C3
• iGEM Parts Registry