Types | DnaRegion
|
Roles | CDS
Coding
|
Sequences | BBa_K1351023_sequence (Version 1)
|
Description
Catalyzes the conversion of uracil and 5-phospho-alpha-D-ribose 1-diphosphate (PRPP) to UMP and diphosphate
Will allow negative selection, Will be Implementet in Modified Plasmid Backbones pSB2E, pSB4S, pSB1C to allow resistance free cloning
This part was generated in a modified version of RFC25, where a strong Shine Dalgarno Sequence (SD) is included, and has the following prefix and suffix:
{|
|prefix with EcoRI, NotI, XbaI, SD and NgoMIV:
|GAATTCGCGGCCGCTTCTAGAG
|-
|suffix with AgeI, SpeI, NotI and PstI:
|ACCGGTTAATACTAGTAGCGGCCGCCTGCAG
|}
Sites of restriction enzymes generating compatible overhangs have the same color:
EcoRI and PstI in blue, NotI in green, XbaI and SpeI in red, NgoMIV and AgeI in orange. Shine-Dalgarno sequence and stop codons are underlined.
Notes
Mutation of Freiburg Standard Restriction Sites within the genomic uppcas was needed
Point Mutation of AgeI restriction site by change of G -> C at position 174
Point Mutation of EcoRI restriction site by change of C -> G at position 477
Source
Derived from pUC19_upp, AG Mascher
Originally from Bacillus subtilis 168 genomic DNA