Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Luna Barroco de Lacerda
Date created: 2014-10-05 11:00:00
Date modified: 2015-07-23 04:05:30
Regulation and transport of mercury ions
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K1355001_sequence (Version 1) |
Description
We call this part an ???Essential biobrick??? designed to be the key piece of any genetic construction related to mercury, targeting both a biosensor as a bioaccumulative. This biobrick has bidirectional promoter, the regulator MerR with a terminator reverse from the tryptophan operon and a forward MerP and MerT transporters.Notes
If is desirable to accumulate mercury, assemble this biobrick with a good RBS, a coding sequence of a peptide having affinity to metal and a terminator! Its function will be regulated by presence of mercury through MerR regulator. The bacteria will carry the mercury to cytoplasm until it is linked to the peptide having affinity to the metal, making a inactive mercury. Is not it amazing? This is the strategy that we use in our project! The so called ???Essential Biobrick??? was connected to biobrick for bioremediation, bioaccumulation and bio-detection!Source
This construction is based on sequences present in the O26-CRL plasmid found in Escherichia coli O26. We added a reverse tryptophan operon terminator to ensure transcription termination of the merR messenger RNA. However we didn???t add transcription terminator after the merP gene, aiming connect this biobrick with others, enabling various functions related to mercury| Sequence Annotation | Location | Component / Role(s) |
| Trp Terminator MerR Bidirecional promoter Bidirecional promoter MerT RBS MerP RBS RBS | 1,43 44,480 486,514 510,538 551,900 901,907 914,1189 490,496 537,543 | sequence_feature feature/misc CDS feature/cds feature/promoter promoter promoter feature/promoter CDS feature/cds feature/rbs ribosome_entry_site feature/cds CDS ribosome_entry_site feature/rbs feature/rbs ribosome_entry_site |