Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Matthew Tucker
Date created: 2014-10-08 11:00:00
Date modified: 2015-05-08 01:10:02
Manganese Peroxidase Expression Vector
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1357006_sequence (Version 1) |
Description
Manganese peroxidase (MnP) is an enzyme from the fungus, Phanerochaete chrysosporium. This enzyme has been shown to degrade nylon 6 and nylon 66. The sequence for MnP in this BioBrick has been codon optimized for E.coli. A pelB secretion tag was also attached to the MnP sequence. The BioBrick also contains a constitutive promoter, a ribosome binding site and a terminator. This BioBrick allows E.coli to secrete manganese peroxidase into the surrounding environment.Notes
The coding gene sequence is optimized for expression in E. coliSource
Commercially sequenced but amino acid sequence taken from Phanerochaete chrysosporium.| Access | Instance | Definition |
| public public public public | BBa_J23100 BBa_B0034 BBa_K1357001 BBa_B0015 | BBa_J23100 BBa_B0034 BBa_K1357001 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23100 BBa_B0034 BBa_K1357001 BBa_B0015 | 1,35 44,55 64,1202 1211,1339 | BBa_J23100 BBa_B0034 BBa_K1357001 BBa_B0015 |