BBa_K136003

BBa_K136003 Version 1

Component

This part has been discontinued.

Source:
http://parts.igem.org/Part:BBa_K136003
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Cyprien Maisonnier
Date created: 2008-08-11 11:00:00
Date modified: 2015-05-08 01:10:02

Promoter of flhB gene from E. coli flagella



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_K136003_sequence (Version 1)

Description

flhB is a class 2 gene that is part of the construction of the flagella ( Ordering Genes in a Flagella Pathway by Analysis of Expression Kinetics from Living Bacteria - S. Kalir, J. McClure, K. Pabbaraju, C. Southward, M. Ronen, S. Leibler, M. G. Surette, U. Alon ).

Its promoter is consitutively repressed.

It is activated by FlhD4C2 and maybe by FliA with different strength ( Using a Quantitative Blueprint to Reprogram the Dynamics of the Flagella Gene Network - Shiraz Kalir and Uri Alon ).

Work in progress : Deeper quantitative characterization

Notes

The primers used to amplify this sequence are :

*Forward :

GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCCACGTCATATCAGGCGGTCTGATAAGG
*Reverse :

GTTTCTTCCTGCAGCGGCCGCTACTAGTAGTTTTGTCGTCGCTCTCGTCAGACACGTC

Source

This part comes from the genome of E.coli K12 strain MG1655.

Sequence Annotation Location Component / Role(s)
FlhDC Binding site 1
FlhDC Binding site 2
Initiation for Transcription
79,94
106,121
153,153
non_covalent_binding_site feature/binding
non_covalent_binding_site feature/binding
sequence_feature feature/misc
igem#experience
None
 
igem#sampleStatus
Discontinued
igem#status
Deleted
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K136003/1