BBa_K136063

BBa_K136063 Version 1

Component

This part has been discontinued.

Please consider using BBa_S04135 as a replacement.

Source:
http://parts.igem.org/Part:BBa_K136063
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Beno??t D
Date created: 2008-10-28 12:00:00
Date modified: 2015-05-08 01:10:03

fliL promoter + WT RBS ( K136009) - EnvZ*



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K136063_sequence (Version 1)

Description

EnvZ is a transmembrane histidine kinase that, in response to osmotic changes, phosphorylates OmpR the natural inhibitor of flhDC promotor the master regulator of the flagella synthesis. EnvZ* is here a mutated version which constitutively phosphorylates OmpR.
fliL is a class 2 gene that is part of the construction of the flagella ( Ordering Genes in a Flagella Pathway by Analysis of Expression Kinetics from Living Bacteria - S. Kalir, J. McClure, K. Pabbaraju, C. Southward, M. Ronen, S. Leibler, M. G. Surette, U. Alon, ).

Its promoter is consitutively repressed.

It is activated by flhD4C2 and by fliA with different forces ( Using a Quantitative Blueprint to Reprogram the Dynamics of the Flagella Gene Network - Shiraz Kalir and Uri Alon ).


This construction may allow ,with the add of a reporter tag, to see some oscillations

Notes

Assembled biobricks

Source

the two parts of this construction were obtained by cloning from the genome of MG1655 WT and mutated for EnvZ bacteria strain

igem#experience
None
 
igem#sampleStatus
Discontinued
igem#status
Deleted
 
isReplacedBy
BBa_S04135/1
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K136063/1