BBa_K1361994

BBa_K1361994 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1361994
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shoujie Sun
Date created: 2014-10-05 11:00:00
Date modified: 2015-05-08 01:10:04

Curli Fiber major monomer-CsgA with his tag



    • Details

    Types
    DnaRegion

    Roles
    CDS

    Coding

    Sequences BBa_K1361994_sequence (Version 1)

    Description

    This part is CsgA(BBa_K1361999) with twin 7Xhis tag. And can be used for purified of CsgA monomer or absorbing Au-NTA-Ni nano partical.
    CsgA is the major monomer of curli fiber. In vivo, its alone has little polymerizability and CsgB protein is requested for quick polymerization of CsgA.

    Notes

    The modifying of CsgA was referring the supplementary of Lu, Michelle Y., et al. "Synthesis and patterning of tunable multiscale materials with engineered cells." (2014).

    Source

    CsgA-his was synthesized by GeneScrip.

    Sequence Annotation Location Component / Role(s)
    cds
    7XHis
    7XHis
    		1,498
    127,147
    475,495
    		CDS feature/cds
    feature/tag tag
    feature/tag tag

    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1361994/1
     

    Attachments

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