BBa_K1361999

BBa_K1361999 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1361999
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shoujie Sun
Date created: 2014-10-02 11:00:00
Date modified: 2015-05-08 01:10:04

Curli Fiber major monomer-CsgA



    • Details

    Types
    DnaRegion

    Roles
    polypeptide_domain

    Protein_Domain

    Sequences BBa_K1361999_sequence (Version 1)

    Description

    CsgA is the major monomer of curli fiber, molecular weight of which is 15.049 kD (from nucleotide sequence) . It has been code modified for biobricks standard. In vivo, its alone has little polymerizability and CsgB protein is requested for quick polymerization of CsgA.

    Notes

    The PstI loci within CsgA sequence has been replaced from CTGCAG to CTGCCG.

    Source

    PCR on genomic DNA from E coli. DH5alpha strain. And site-directed mutation by overlap PCR primers.

    Sequence Annotation Location Component / Role(s)
    CsgA
    start
    stop
    		1,456
    1,3
    454,456
    		feature/cds CDS
    start_codon feature/start
    feature/stop stop_codon

    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    rdf-schema#seeAlso
    P28307
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1361999/1
     

    Attachments

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