Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Manon Molina
Date created: 2014-09-30 11:00:00
Date modified: 2015-06-08 08:39:49
RBS - Antifungal GAFP-1
| Types | DnaRegion |
| Roles | Translational_Unit engineered_region |
| Sequences | BBa_K1364002_sequence (Version 1) |
Description
The Gastrodia anti-fungal protein (GAFP-1) is a mannose-binding lectin which is able to inhibit the growth of multiples species of plant pathogenic fungi.The RBS used is a strong RBS for Bacillus subtilis (K780002).
Notes
This part is composed of a Strong RBS (K780002) and the open reading frame of the Gastrodia anti-fungal protein 1 (GAFP-1) optimized for its expression and its secretion in Bacillus subtilis. The codon optimization was made thanks to the DNA 2.0 software program.Source
This gene was ordered from a synthesis company.| Sequence Annotation | Location | Component / Role(s) |
| strong RBS K780002 Signal peptide Propeptide GAFP-1 | 1,16 17,97 98,139 140,187 | feature/rbs ribosome_entry_site CDS feature/protein CDS feature/protein feature/cds CDS |