Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jourdan Camille
Date created: 2014-09-24 11:00:00
Date modified: 2016-02-03 10:04:36
RBS - Antifungal D4E1 - Double terminator
| Types | DnaRegion |
| Roles | Translational_Unit engineered_region |
| Sequences | BBa_K1364003_sequence (Version 1) |
Description
D4E1 is a linear synthetic peptide of 17 amino acids which has shown to have antifungal activities by complexing with a sterol present in conidial wall of a variety of fungi.This part is composed of a RBS (K780002 spoVG), the open reading frame of D4E1 and a double terminator (B0015). This part was optimized for the expression and its secretion in Bacillus subtilis.
Notes
The coding sequence of D4E1 is flanked on the N-terminal end with a signal peptide (amyE signal peptide) followed by a pro peptide, cleaved during the secretion process.Source
De Lucca AJ,??Bland JM,??Grimm C,??Jacks TJ,??Cary JW,??Jaynes JM,??Cleveland TE,??Walsh TJ. Fungicidal properties, sterol binding, and proteolytic resistance of the synthetic peptide D4E1.Can J Microbiol.??1998 Jun;44(6):514-20.
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K780002 strong RBS Signal Peptide Pro Peptide D4E1 BBa_B0015 | 1,16 17,97 98,139 140,192 194,322 | ribosome_entry_site feature/rbs CDS feature/protein CDS feature/protein feature/cds CDS engineered_region feature/BioBrick |