BBa_K1365003

BBa_K1365003 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1365003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Sandra Mous

Date created: 2014-09-23 11:00:00

Date modified: 2015-05-08 01:10:07

NisC

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K1365003_sequence (Version 1)

Description

NisC catalyzes the ring formation in the nisin precursor protein. Together with the genes NisA, NisB, NisT, NisP, NisR and NisK it is responsible for producing the lantibiotic nisin in Lactococcus lactis.

Notes

Used primers:
forward - 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAATAAAAAAAATATAAA-3'
reverse - 5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATCATTTCCTCTTCCCTC-3'
to PCR NisC from the genome and add the BioBrick prefix and suffix.

Source

Obtained by PCR on the genome of Lactococcus lactis NZ9700, a nisin producing strain.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
start	1,3	feature/start, start_codon
stop	1243,1245	feature/stop, stop_codon
NisC	1,1245	feature/cds, CDS

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1365003/1

Member of these Collections

• iGEM Parts Registry