BBa_K1365004

BBa_K1365004 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1365004

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Sandra Mous

Date created: 2014-09-23 11:00:00

Date modified: 2015-05-08 01:10:07

NisI

Details

• Types: DnaRegion
• Roles: Coding; CDS

Description

NisI is a nisin immunity gene. In Lactococcus lactis it protects against it's own nisin.

Notes

Used primers:
forward - 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGAAGATATTTAATACT-3'
reverse - 5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTATTACTAATTTCCTACCTTCG-3'
to PCR NisI from the genome and add the BioBrick prefix and suffix.

Deleted illegal SpeI site at position 762 using above primers, changing a AAC codon in a AAT.

Source

Obtained by PCR on the genome of Lactococcus lactis NZ9700, a nisin producing strain.

Other Properties

• igem#sampleStatus: No part sequence
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1365004/1

Member of these Collections

• iGEM Parts Registry