BBa_K1365007

BBa_K1365007 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1365007

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Sandra Mous

Date created: 2014-09-23 11:00:00

Date modified: 2015-05-08 01:10:07

NisF, NisE and NisG

Details

• Types: DnaRegion
• Roles: engineered_region; Composite

Description

Together with NisI, the genes NisF, NisE and NisG provide immunity against nisin for Lactococcus lactis.

Notes

Used primers:
forward - 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGCAGGTAAAAATTCAAAA-3'
reverse - 5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATCTAATCTTTTTTT-3'
to PCR NisF, NisE and NisG from the genome and add the BioBrick prefix and suffix.

The illegal PstI site at position 186, illegal EcoRI site at postion 336 and illegal XbaI site at position 349 were removed using primers as well, fusing the resulting PCR fragments together again with a Gibson assembly.

Source

Obtained by PCR on the genome of Lactococcus lactis NZ9700, a nisin producing strain.

Other Properties

• igem#sampleStatus: No part sequence
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1365007/1

Member of these Collections

• iGEM Parts Registry