BBa_K1365009

BBa_K1365009 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1365009

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Sandra Mous

Date created: 2014-09-23 11:00:00

Date modified: 2015-05-08 01:10:07

PNisI with RBS

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K1365009_sequence (Version 1)

Description

Promoter with Original RBS from Lactococcus lactis NZ9700. Activated by NisR. Normally placed in front of the NisA gene, for regulating transcription of the nisin precursor.

Notes

Used primers:
forward - 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAGCACTGGATAATGACTATT-3'
reverse - 5'-TACTAGTAGCGGCCGCTGCAGGAAGAAACTTCCTCTTCCCTCCTTTCAA-3'
to PCR PNisI from the genome and add the BioBrick prefix and suffix.

Source

Obtained by PCR on the genome of Lactococcus lactis NZ9700, a nisin producing strain.

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1365009/1

Member of these Collections

• iGEM Parts Registry