Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lisa Hielkema
Date created: 2014-10-07 11:00:00
Date modified: 2015-05-08 01:10:07
pIL253-mRFP Biobrick Vector for L.lactis
| Types | DnaRegion |
| Roles | Plasmid_Backbone plasmid_vector |
| Sequences | BBa_K1365301_sequence (Version 1) |
Description
This plasmid can be used to transform biobricks in L. lactis. Moreover it shuttles between L. lactis and B. subtilis.It contains mRFP Biobrick Prefix and Sufix. And it has an erythromycin resistance.
Notes
Cut out the multiple cloning sites of pIL253 also removed illegal restriction sites of EcoRI and XbaISource
Yes, Derived from pIL253. Provided by molGen at the university of Groningen.| Sequence Annotation | Location | Component / Role(s) |
| G00101 (VR) G00100 (VF2) J04450 (mRFP Construct) Origin of Replication ORF3 Erythromycin resistance R0010 (LacI reg. promoter) end of LacI coding region (inactive) CAP binding site -35 -10 LacI binding site start B0034 (RBS) start E1010 (mRFP) stop stop B0015 B0010 B0012 prefix suffix start | 1369,1388 6,25 146,1214 1885,3375 4368,4838 4959,5696 146,345 146,233 234,271 282,287 306,311 311,345 318,318 354,365 372,372 372,1046 1050,1050 1047,1047 1086,1214 1087,1143 1181,1200 124,145 1215,1235 5696,5696 | primer_binding_site feature/primer_binding primer_binding_site feature/primer_binding engineered_region feature/BioBrick feature/dna sequence_feature feature/dna sequence_feature feature/cds CDS engineered_region feature/BioBrick feature/cds CDS non_covalent_binding_site feature/binding promoter feature/promoter feature/promoter promoter non_covalent_binding_site feature/binding start_codon feature/start ribosome_entry_site feature/rbs feature/start start_codon feature/cds CDS stop_codon feature/stop stop_codon feature/stop feature/BioBrick engineered_region feature/stem_loop stem_loop stem_loop feature/stem_loop sequence_feature feature/misc feature/misc sequence_feature feature/start start_codon |