Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Eduardo Cepeda Ca??edo, Mercedes Alejandra Vazquez Cantu
Date created: 2014-10-09 11:00:00
Date modified: 2015-05-08 01:10:07
Apoptin expression under a CMV promoter (C5)
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1366104_sequence (Version 1) |
Description
This construct will be used to characterize the transfection of a death effector (apoptin) in mammalian cells mediated by bacteriophages. It contains the hTERT promoter, specific for cancerous cells lines. The construct will be contained in a phagemid with de f1 origin of replication (for single stranded DNA), and it is intended to replace the M13 genome. Apoptin will be codon optimized for mammalian cell line.Notes
The sequence design goes like this CMV Promoter+RBS // Apoptin // terminatorSource
Yes, we use NCBI to retrieve the DNA sequence. The accession number is NC_001427.1| Sequence Annotation | Location | Component / Role(s) |
| CMV promoter + RBS BBa_I712004 hGH terminator BBa_K404108 Apoptin | 1,654 1,654 1041,1522 1041,1522 655,1040 | feature/promoter promoter engineered_region feature/BioBrick sequence_feature feature/misc feature/BioBrick engineered_region feature/misc sequence_feature |