Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Eduardo Cepeda Ca??edo, Mercedes Alejandra Vazquez Cantu
Date created: 2014-10-09 11:00:00
Date modified: 2015-05-08 01:10:08
Apoptin and survivin siRNA production (C9)
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1366108_sequence (Version 1) |
Description
This construct will be used to characterize the transfection of two effectors of death (Survivin siRNA and apoptin) in mammalian cells mediated by bacteriophages. The survivin siRNA is expressed under an hTERT promoter, specific for cancerous cells lines. The apoptin is expressed under a CMV promoter because it is extremely specific, so it wouldn't harm the normal cells.The construct will be contained in a phagemid with de f1 origin of replication (for single stranded DNA), and it is intended to replace the M13 genome.
Notes
The construct structure is as follows: CMV // Apoptin // terminator // hTERT // siRNA // terminatorSource
The apoptin DNA sequence was retrieved from NCBI with the accession number of NC_001427.1. The siRNA was designed by us. And the rest of the parts were from the registry.| Sequence Annotation | Location | Component / Role(s) |
| CMV + RBS hGH polyadenylation Apoptin hGH polyadenylation hTERT survivin siRNA 1 | 1,654 2015,2496 655,1020 1021,1502 1503,1962 1963,2014 | promoter feature/promoter stop_codon feature/stop CDS feature/cds feature/stop stop_codon feature/promoter promoter sequence_feature feature/dna |