BBa_K1369000

BBa_K1369000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1369000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Date created: 2014-08-17 11:00:00

Date modified: 2015-05-08 01:10:08

Repressor-Repressor promoter (TetR and LacI operator sites)

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K1369000_sequence (Version 1)

Description

This is a repressor-repressor (RR) combinatorial promoter which consists of a LacI operator site upstream of the -35 RNA polymerase binding site, and a TetR operator site within the -35 and -10 RNA polymerase boxes.

Notes

To test the logic of this RR promoter, we assembled constructs containing the promoter, a bicistronic RBS (part BBa_J119024), and GFP, in that order, downstream of the promoter.

Source

This part is taken from the paper "Programming gene expression with combinatorial promoters," by Robert Sidney Cox III et al.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
LacI operator site	70,102	feature/operator, operator
TetR operator site	45,67	feature/operator, operator
-35 RNA polymerase box	40,45	sequence_feature, feature/misc
-10 RNA polymerase box	63,68	sequence_feature, feature/misc

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1369000/1

Member of these Collections

• iGEM Parts Registry