BBa_K1369001

BBa_K1369001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1369001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Date created: 2014-08-17 11:00:00

Date modified: 2015-05-08 01:10:08

Repressor-Repressor promoter (LacI and TetR operator sites)

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_K1369001_sequence (Version 1)

Description

This is a repressor-repressor (RR) combinatorial promoter which consists of a TetR operator site within the -35 and -10 RNA polymerase boxes and a LacI operator site downstream of the -10 RNA polymerase box.

Notes

To test the logic of this RR promoter, we assembled constructs containing the promoter, a bicistronic RBS (part BBa_J119024), and GFP, in that order, downstream of the promoter.

Source

This part is taken from the paper "Programming gene expression with combinatorial promoters," by Robert Sidney Cox III et al.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
LacI operator site	1,42	operator, feature/operator
-35 RNA polymerase box	39,43	feature/misc, sequence_feature
-10 RNA polymerase box	62,66	sequence_feature, feature/misc
TetR operator site	45,66	feature/operator, operator

Other Properties

• igem#experience: Works
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1369001/1

Member of these Collections

• iGEM Parts Registry