BBa_K137013

BBa_K137013 Version 1

Component

This part has been discontinued.

Source:
http://parts.igem.org/Part:BBa_K137013
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Victoria Hsiao
Date created: 2008-06-19 11:00:00
Date modified: 2015-05-08 01:10:08

folC (folylpolyglutamate synthase + dihydrofolate synthase)



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K137013_sequence (Version 1)

Description

folC is a necessary gene in the L.lactis folate biosynthesis gene cluster. folC is a bifunctional gene which encodes both folylpolyglutamate synthase and dihydrofolate synthase. These enzymes are needed for the last steps of dihydrofolate production.

Notes

We were unsure of whether or not the L.lactis RBS would work with E. coli and so we are going to try cloning the entire gene cluster with the RBSs as well as cloning out each gene separately and adding E.coli RBSs.

Source

L.lactis genome

Sequence Annotation Location Component / Role(s)
EcoR1 site
EcoR1 site
860,860
979,979
feature/s_mutation point_mutation
feature/s_mutation point_mutation
igem#experience
None
 
igem#sampleStatus
Discontinued
igem#status
Deleted
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K137013/1