| Types | DnaRegion
|
| Roles | Coding
CDS
|
| Sequences | BBa_K137017_sequence (Version 1)
|
Description
Galactose oxidase is a fungal enzyme that uses molecular oxygen to oxidizes galactose and several other primary alcohols to the corresponding aldehyde, while also producing hydrogen peroxide. This particular galaxtose oxidase is not wild type. It has been optimized through directed evolution by the Arnold lab at CalTech to be functionally expressed in vivo in E. coli and to be thermostable. (Sun L, Petrounia IP, Yagasaki M, Bandara G, and Arnold FH. Expression and stabilization of galactose oxidase in Escherichia coli by directed evolution. Protein Eng 2001 Sep; 14(9) 699-704. pmid:11707617). This parts has undergone two rounds of site directed mutagenesis to remove one EcoRI and one PstI site. In our hands, it is functional towards galactose in vivo.
Notes
Two rounds of site directed mutagenesis had to be performed to eliminate an EcoRI and PstI site.
Source
Plasmid from the Arnold lab at CalTech, which is the result of their directed evolution to make galactose functionally expressed in E. coli and to improve its thermostability.
