BBa_K137046

BBa_K137046 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K137046
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Allen Lin
Date created: 2008-07-20 11:00:00
Date modified: 2015-05-08 01:10:09

150 bp inverted tetR promoter



    • Details

    Types
    DnaRegion

    Roles
    promoter

    Regulatory

    Sequences BBa_K137046_sequence (Version 1)

    Description

    tetR promoter with noncoding, spacer DNA upstream of it to make the total length 150 bp

    Notes

    This part is in a series of tetR promoter that have total lengths of 150, 250, 350, 450, 650, and 850 bp. We chose the region upstream of tetR in part Q04400 to be the noncoding DNA segment. This noncoding segment consists of the 3' end of tetR and B0015. None of the parts in this series was long enough to include the start codon of tetR, so a functional tetR protein should not be transcribed.

    Source

    Primers were synthesized to bind to part Q04400, and then a PCR reaction was run.

    Sequence Annotation Location Component / Role(s)
    tetR promoter
    		1,54
    		promoter feature/promoter

    igem#experience
    Works
     
    igem#sampleStatus
    It's complicated
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K137046/1
     

    Attachments

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