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BBa_K137056

BBa_K137056 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K137056

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Victoria Hsiao

Date created: 2008-07-30 11:00:00

Date modified: 2015-05-08 01:10:09

folB + folKE + b0015 in psb2k3

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Details

Types	DnaRegion
Roles	engineered_region Composite
Sequences	BBa_K137056_sequence (Version 1)

Description

This is the complete construct of two of the five folate biosynthesis genes from L.lactis. We hope to use this plasmid to test the effects of overexpression of the folate gene cluster on total folate production in E. coli.

Notes

The rest of the plasmid includes the constitutive promoter j23100 and the strong RBS b0034 in the inducible copy plasmid psb2k3.

Source

The two genes were cloned from the genome of lactococcus lactis subspe. IL1403

Components

Access	Instance	Definition
public public public public public public public	BBa_J23100 BBa_B0034 BBa_K137009 BBa_B0034 BBa_K137011 BBa_B0010 BBa_B0012	BBa_J23100 BBa_B0034 folB BBa_B0034 folKE BBa_B0010 BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_J23100 BBa_B0034 BBa_K137009 BBa_B0034 BBa_K137011 BBa_B0010 BBa_B0012	1,35 44,55 62,415 424,435 442,1494 1503,1582 1591,1631	BBa_J23100 BBa_B0034 folB BBa_B0034 folKE BBa_B0010 BBa_B0012

Other Properties

igem#experience	None
igem#sampleStatus	Not in stock
igem#status	Unavailable
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_K137056/1