Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Doug Tischer
Date created: 2008-08-18 11:00:00
Date modified: 2015-05-08 01:10:09
AHL -> H2O2
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K137078_sequence (Version 1) |
Description
This is the final construct from one of the projects of Caltech 2008 iGEM team. In response to being induced with acylhomoserine lactone, the construct should cause the E. coli to produce pyruvate oxidase (SpxB) while simulataneously shutting down production of a catalase, KatG. KatG is cleared rapidly from the cell due to an LAA degradation tag, allowing hydrogen peroxide produced from the pyruvate oxidase to build to bactericidal concentrations. Preliminary data suggests pyruvate oxidase can produce 0.8 mM hydrogen peroxide in the media supernatant.Notes
None.Source
Genomic PCR for the pyruvate oxidase (from Streptococcus pnumoniae R6) and catalase (katG from E. coli DH10B), while the rest of the construct is made out of parts from the registry.| Access | Instance | Definition |
| public public | BBa_S04057 BBa_S04059 | BBa_S04057 BBa_S04059 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_S04057 BBa_S04059 | 1,3797 3804,6157 | BBa_S04057 BBa_S04059 |