BBa_K137127

BBa_K137127 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_K137127

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Robert Ovadia

Date created: 2008-10-15 11:00:00

Date modified: 2015-05-08 01:10:11

A81 Lac Promoter - Tight expression levels

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_K137127_sequence (Version 1)

Description

A lactose inducible promoter obtained from: Cox RS III, Surette MG, Elowitz MB. Programming gene expression with combinatorial promoters. Mol. Syst. Biol. 2007;3:145.
Used for tight expression levels to maintain our lysis cassette genes. This allows the cell to lyse only when lactose is present, as for lysis at any other time may cause problems in our system.

Notes

Ordered primers to PCR them out of the original plasmid, but they failed.
Decided to order long primers and "dimerized" the promoter with restriction sites at the ends.

Source

Cox RS III, Surette MG, Elowitz MB. Programming gene expression with combinatorial promoters. Mol. Syst. Biol. 2007;3:145.

Other Properties

• igem#experience: None
• igem#sampleStatus: Discontinued
• igem#status: Deleted
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K137127/1

Member of these Collections

• iGEM Parts Registry