| Types | DnaRegion
|
| Roles | Regulatory
promoter
|
| Sequences | BBa_K137128_sequence (Version 1)
|
Description
An additional lactose inducible promoter (similar to A81) obtained from: Cox RS III, Surette MG, Elowitz MB. Programming gene expression with combinatorial promoters. Mol. Syst. Biol. 2007;3:145. Used for tight expression levels to maintain our lysis cassette genes. This allows the cell to lyse only when lactose is present, as for lysis at any other time may cause problems in our system.
Notes
Ordered long primers and "dimerized" the promoter with restriction sites at the ends.
Source
Cox RS III, Surette MG, Elowitz MB. Programming gene expression with combinatorial promoters. Mol. Syst. Biol. 2007;3:145.
