BBa_K1378005

BBa_K1378005 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1378005

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Meng Liuyi & Zhang Zijian

Date created: 2014-10-02 11:00:00

Date modified: 2015-05-08 01:10:12

INPNC-linker2-MVN-linker1-mRFP

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K1378005_sequence (Version 1)

Description

This composite part is fused by INP,MVN and mRFP.It can display MVN protein on the surface of E.coli through INP and emission red fluorecence. We use this part to detect whether MVN is translocated on the out membrane.

Notes

we set a TEV protease cutting site into linker2

Source

We fused K523013, K1378005 and mRFP.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
MVN	1007,1330	CDS, feature/cds
J23105	1,35	feature/promoter, promoter
B0034	44,55	feature/rbs, ribosome_entry_site
INPNC	62,985	CDS, feature/cds
linker2	986,1006	feature/misc, sequence_feature
linker1	1331,1360	feature/misc, sequence_feature
mRFP	1361,2041	CDS, feature/cds
B0015	2048,2176	stop_codon, feature/stop

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1378005/1

Member of these Collections

• iGEM Parts Registry