Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Zhang Zijian & Meng Liuyi
Date created: 2014-10-05 11:00:00
Date modified: 2015-05-08 01:10:12
INPNC-MVN & GFP (the Binding Plasmid)
| Types | DnaRegion |
| Roles | Project engineered_region |
| Sequences | BBa_K1378007_sequence (Version 1) |
Description
This plasmid is the final testing plasmid for our E. coli- M. aeruginosa binding assay. We inserted two transcription units into one plasmid and they are expressed under the promoters J23105 and J23117 respectively. After this plasmid is transformed into E. coli, the mannan binding protein MVN (Microvirin) will be displayed on the cell surface by INPNC (Ice Nucleation Protein N and C terminal) and the E. coli cell will express GFP as well. In this way, the E. coli cells will be able to bind to M. aeruginosa cells in a "visible" way.Notes
We optimized the sequence of MVN beforehand and chose two different promoters to avoid possible homologous recombination.Source
This is a composite part so we constructed it using various different smaller parts. INPNC comes from the existing part BBa_K523013. The sequence of MVN can be found in the genomic sequence of Microcystis aeruginosa PCC7806 and the gene was synthesized by a company (sequence can be found in the part BBa_K1378003). GFP is common and comes from the existing part BBa_E0040.| Sequence Annotation | Location | Component / Role(s) |
| B0034 J23105 INPNC Linker1 MVN B0015 J23117 B0030 GFP B0015 | 44,55 1,35 62,985 986,1015 1016,1345 1352,1480 1480,1523 1532,1546 1553,2272 2281,2409 | feature/rbs ribosome_entry_site feature/promoter promoter feature/cds CDS feature/misc sequence_feature CDS feature/cds feature/stop stop_codon promoter feature/promoter ribosome_entry_site feature/rbs CDS feature/cds feature/stop stop_codon |