| Types | DnaRegion
|
| Roles | plasmid
Plasmid
|
| Sequences | BBa_K138000_sequence (Version 1)
|
Description
The NifH promoter was subcloned into the leucine landing pad using Xba and Nde. Other genetic elements can be subcloned behind the promoter using either NdeI/SpeI or NdeI/PstI (or other combinations). The linker region is the following: EcorI-XbaI- NifHp- NdeI- PacI-SpeI-PstI.
Notes
Landing pad has ampicillin and chloramphenicol resistance; see protocol on Michigan's wiki on how to get the things in the landing pad on the chromosome
Source
The Leucine Landing Pad was built by Dong Eun Chang, a former member of the Ninfa Lab. nifHp was a PCR product, with the template being pRT22, a nifH-lacZ recorder plasmid. This construct was provided to us by Dr. Ray Dixon of the John Innes Center in the United Kingdom.
